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. Author manuscript; available in PMC: 2012 Feb 6.
Published in final edited form as: J Alzheimers Dis. 2010;22(2):683–984. doi: 10.3233/JAD-2010-100816

Figure 7. Validation of altered APP processing with changes in cell density.

Figure 7

A-B, H4-APP751 cells were seeded on 10cm-plates with different densities (0.5M, 1M, 1.5M, 2M, and 3M; equivalent to the densities of 100K, 200K, 250K, 300K, and 400K in 6-well plates, respectively). Cell lysates were collected after 48h and were applied to the BCA assay to determine protein concentration and the fluorometric assay to measure α- or β-secretase activity. The fluorometric readout was normalized to the protein concentration for each sample. Normalized fluorometric values at higher cell densities were compared to those at 100K. At higher seeding densities, the APP processing activity of α- and β-secretase decreased when compared to those at 100K (n=3 for each treatment group; mean ± S.E.; *p<0.05; **p<0.01).