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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Arch Biochem Biophys. 2011 Dec 16;518(1):79–88. doi: 10.1016/j.abb.2011.12.006

Figure 2. p53 knock down causes decrease in growth rate in cancer cell lines.

Figure 2

A–C Mutant p53 levels were knocked down in three cancer cell lines by lentivirus expressing p53 shRNA as indicated and their growth rates measured in comparison to the corresponding cell lines generated after infecting with lentivirus expressing GFP shRNA as described in Materials and methods. Cells were harvested every other day and cell numbers were determined by Coulter Counter. Error bars represent standard deviation between the average cell counts. Experiments were done in triplicate, and data are representative of multiple independent repeats. Immunoblots show the efficacy of p53 knock down. ERK2 was used as a loading control. D. For the H1048 cells, we performed a PARP cleavage assay to check for caspase activation using PARP antibody from Cell Signaling. E. Apoptosis DNA-Ladder assay of H1048 cells stably transfected with shp53 (1) or shGFP (2). U937 cells after treatment with CAM (3) were used as a positive control for apoptosis.