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. Author manuscript; available in PMC: 2012 Feb 6.
Published in final edited form as: Nat Immunol. 2011 Mar 20;12(5):441–449. doi: 10.1038/ni.2011

Figure 1. Identification and initial characterization of strains with reduced B lymphocytes after ENU-induced mutagenesis.

Figure 1

(a) Flow cytometric analysis of the percentage of B220+ B cells in the blood of Atp11c+/0 wild-type (WT), Atp11camb/0 ambrosius (Amb) and 18NIH30a male mice. All data are expressed as a percentage of lymphocytes. Each white circle (WT) and black circle (Amb) represents a single mouse. (b) Primary antibody response 14 days after immunization with inactivated Bordetella pertussis (BP) and alum-precipitated CGG (left). The other panels show antibody to CGG, the hapten ABA, and NP-Ficoll 6 days after booster immunization. Each white circle (WT) and black circle (Amb) represents a single mouse. (c) Number of lymphocytes, neutrophils, reticulocytes and erythrocytes in the blood of Amb and WT littermates. The results are representative of 2 independent experiments with 4–7 mice per group in each experiment. (d) Unconjugated and conjugated bilirubin in the plasma of Amb and control mice. The data are representative of 2 independent experiments with 4 to 7 mice per group and experiment. (e) Typical dysplastic focus on H & E stained liver section from amb mice at 6 months (X400).