Table 1. Number of B cells in the spleen and B cell subpopulations in bone marrow.

The numbers are expressed as mean values x10⁵ ± S.E.M in the bone marrow and x10⁶ ± S.E.M in the spleen. B cells (B220⁺ in live lymphocytes gate) in the spleen. Mature B cell (IgD⁺ IgM⁺ in B220⁺ gate), immature B cells (IgD⁻ IgM⁺ in B220⁺ gate), pre-B cell (CD24^hi CD43⁻ in IgD⁻ IgM⁻ gate), pro-B cell (CD24^int CD43⁺ in IgD⁻ IgM⁻ gate), pre-pro-B cell (CD24⁻CD43⁺ in IgD⁻ IgM⁻ gate) in the bone marrow.

ATP11c	Transgene	Pre-Pro-B cells	Pro-B cells	Pre-B cells	Immature B cells	Mature B cells	Number of animals (BM)	Spleen B cells	Number of animals (Spleen)
WT	None	3.99 ± 0.25	4.10 ± 0.42	28.66 ± 2.04	12.50 ± 1.20	14.29 ± 0.89	14	20.06 ± 1.87	11
Amb	None	5.55 ± 0.36	2.22 ± 0.42	1.74 ± 0.34	0.24 ± 0.04	1.75 ± 0.25	13	1.73 ± 0.20	10
WT	vavBcl2	5.53 ± 0.48	6.48 ± 0.65	21.48 ± 5.72	17.60 ± 1.65	69.63 ± 13.55	4	101.10 ± 11.84	4
Amb	vavBcl2	6.22 ± 0.49	4.78 ± 0.96	6.22 ± 1.37	3.12 ± 0.26	9.98 ± 1.06	6	6.18 ± 1.37	6
WT	IL-7	2.36 ± 0.56	19.26 ± 2.28	262.00 ± 36.5	36.36 ± 3.86	1.66 ± 0.31	5	153.30 ± 18.25	3
Amb	IL-7	5.33 ± 0.21	2.33 ± 0.40	1.68 ± 0.43	0.25 ± 0.03	6.30 ± 1.45	4	7.78 ± 1.94	2
WT	MD4	2.53 ± 0.29	0.50 ± 0.06	0.73 ± 0.13	15.97 ± 0.89	10.07 ± 0.84	3	10.82 ± 1.64	3
Amb	MD4	3.28 ± 0.32	0.70 ± 0.03	0.66 ± 0.03	1.98 ± 0.39	4.90 ± 1.04	5	3.00 ± 0.23	5