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(a) Induction of Deltex1 and Hes1 expression in PD1.6 cells overexpressing both ICN-Notch1 and Bcl-2. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis of the indicated cells. GAPDH was used as loading control. (b) Western blot analysis of Bcl-2 and Notch1 expression in PD1.6, PD1.6Bcl-2, PD1.6Notch1, and PD1.6Bcl-2Notch1 cells. α-Tubulin was used as loading control.
