Figure 2.

Notch pathway was activated in the glomeruli of Akita diabetic mice and telmisartan inhibited its expression. The expression of the intracellular domain of Notch1 (ICN1) (a and c), Jagged1 (d), and transforming growth factor β (TGF-β) (e and f) were examined by immunohistochemistry. Anti-podocalyxin (Podxl) antibody was used as a marker for podocyte. ICN-1 was localized to podocyte nuclei (c), while TGF-β was localized to podocyte cytoplasm, respectively (f). Quantification of ICN1-positive cells per glomeruli was performed (b). Ten glomeruli of each specimen were randomly selected. The ICN1-positive cells within the glomeruli were counted under a fluorescence microscope. Statistical significance was analyzed using Student's t-test. Arrows indicated the glomerulus. Bars indicated the mean value. **P < 0.01.