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Intact cell labeling of legumain with fluorescently labeled inhibitors. (a) Structures of Cy5-labeled legumain probes (b) RAW 264.7 macrophages were treated with Cy5-labeled legumain inhibitors for 60 min at different concentrations. After incubation, cells were lysed under hypotonic conditions and all labeled proteins were separated by 12.5% SDS-PAGE and analyzed by scanning the gel with a Typhoon flatbed scanner.
