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. Author manuscript; available in PMC: 2012 Nov 29.
Published in final edited form as: Biochemistry. 2011 Nov 2;50(47):10328–10342. doi: 10.1021/bi201371j

Figure 4.

Figure 4

Titration of U-15N-Aβ42 with sulindac sulfone. (A) 15N-HSQC spectra of Aβ42 upon titration of sulindac sulfone at concentrations ranging from 0 to 500 μM. There are no shifts in the peaks of these spectra beyond what is observed for the DMSO-only control titration (see Fig. 6) and peak intensities do not vary. (B) 1H NMR spectra taken at each titration point to allow observation of ligand peaks throughout the titration. It can be seen that the sulindac sulfone peaks remain sharp throughout, reflecting the fact that this compound does not aggregate at concentrations below 500 uM. (C) 1-D 1H NMR projections of the HSQC spectra shown in panel A demonstrate that the solubility of Aβ42 remains unchanged at all points.