Figure 5.

LRP1 regulates Aβ endocytosis in N2a cells. A, N2a–mLRP4 cells were incubated with 10 nm [¹²⁵I]RAP (filled circles) or 100 nm [¹²⁵I]Aβ₄₂ (open circles) for 1 h at 4°C and then warmed to 37°C for the indicated times. B, N2a–pcDNA3 and N2a–mLRP4 cells were similarly treated with 100 nm [¹²⁵I]Aβ₄₂. The percentage of ligand internalized at each time interval is equal to the amount of ligand internalized divided by the total cell-associated ligand. Error bars indicate SD from three independent experiments. **p < 0.01.