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Representative Western blot with a polyclonal antibody directed against PA28 in BAL supernatant of twelve patients with ARDS. PA28 protein could not be detected in the BAL supernatant of ARDS patients. Start and front of the gel were marked as + and −. Lanes are identified as follows: Lane 1: 1 μg PA28 (standard); Lane 2–13 BAL supernatant of twelve ARDS patients.
