Table 3.
Effects of altering PLKA on hyphal length and number of nucleia
| Strain (genotype) | Medium | Hyphal length (μm ± SEM) | No. of nuclei (μm ± SEM) |
|---|---|---|---|
| KM14 (ΔplkA) | YAG | 32.0 ± 1.9 | 6.0 ± 0.3 |
| KM25 (plkA) | YAG | 26.3 ± 1.7 | 5.3 ± 0.2 |
| TN02A25 (plkA) | YAG | 26.8 ± 1.7 | 5.9 ± 0.3 |
| KM17 [alcA(p)::plkA] | YAG | 30.1 ± 2.7 | 5.7 ± 0.3 |
| KM5 (plkA) | YAG | 29.0 ± 2.0 | 5.4 ± 0.3 |
| KM17 [alcA(p)::plkA] | mmTF | 39.1 ± 1.9 | 3.3 ± 0.2 |
| KM5 (plkA) | mmTF | 34.5 ± 1.7 | 3.0 ± 0.1 |
| TN02A25 (plkA) | mmTF | 38.2 ± 2.1 | 3.7 ± 0.2 |
a
Cells were incubated in YAG medium at 32°C for 7 h or in mmTF for 12 h, fixed, and then stained with DAPI. Approximately 50 cells were scored for each strain.