Table 4.
Effects of altering PLKA on spindle mitotic index and spindle and chromosome organization
| Strain (genotype) | Mediuma | SMI (%)b | Spindle pattern (%)c |
Abnormal chromosome pattern (%)d | |
|---|---|---|---|---|---|
| Telophase | Abnormal | ||||
| TN02A25 (plkA) | YAG | 4.2 | 17.6 | 2.0 | 2.0 |
| KM25 (plkA) | YAG | 5.2 | 17.0 | 0 | 3.4 |
| KM14 (ΔplkA) | YAG | 10.7 | 27.0 | 16.4 | 12.7 |
| KM17 [alcA(p)::plkA] | YAG | 9.0 | 19.5 | 17.0 | 9.8 |
| KM5 (plkA) | YAG | 4.5 | 15.5 | 5.1 | 6.9 |
| TN02A25 (plkA) | mmTF | 5.2 | 17.3 | 5.8 | 3.8 |
| KM5 (plkA) | mmTF | 5.6 | 13.8 | 5.2 | 0 |
| KM17 [alcA(p)::plkA] | mmTF | 3.6 | 12.5 | 3.1 | 3.1 |
a
Strains were incubated in YAG for 7 h or in mmTF for 7 h, processed for immunolocalization of α-tubulin, and stained with DAPI.
b
SMI, spindle mitotic index. Data represent the proportion of total cells that contained a spindle. Approximately 300 to 500 cells were scored for each strain.
c
Proportion of spindles in telophase or abnormal in structure. Approximately 50 spindles were scored for each strain.
d
Proportion of mitotic cells that demonstrated abnormal chromosome segregation.