Fig 1.

Single-cell Epa1 expression is heterogeneous and correlates with single-cell adherence phenotype. (A) Flow cytometric analysis of cellular fluorescence from either wild-type C. glabrata BG2 (autofluorescence), C. glabrata expressing an EPA1-GFP transcriptional fusion construct, or S. cerevisiae expressing an ACT1-GFP transcriptional fusion. (B) C. glabrata BG2 cells expressing an Epa1-HA construct were gated and sorted by FACS analysis according to Epa1-HA expression level, as indicated with anti-HA, Alexa Fluor 488 conjugate antibody (see Fig. 3A for example of gating). Sorted cells of the high- and low-Epa1-HA-expressing subpopulations were tested for adherence to HEp-2 epithelial cells, in comparison with unsorted C. glabrata BG2 and the isogenic epa1Δ mutant. Mean data from replicate determinations are shown ± SD.