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Development of 4-plex fluorescent microsphere immunoassay. Each individual corresponding bead set (indicated in each panel) was first tested in singleplex format and then combined for testing in 4-plex format. (A) Oral fluid-based FMIAs; (B) serum-based FMIAs. The correlation coefficient (R2) indicated in each panel was determined for comparison between each singleplex assay and the 4-plex FMIA.
