Table 1.
Oligonucleotide primers used in this studya
| Primer | Sequence (5′–3′) | Note |
|---|---|---|
| P1 | TTGCATCAATACTGGACTC | Inactivation, flgJBb, F |
| P2 | TTGAATTTTCAGATCCCTC | Inactivation, flgJBb, R |
| P3 | AAGCTTTAATACCCGAGCTTCAAG | kan cassette, F |
| P4 | AAGCTTTCAAGTCAGCGTAATGCT | kan cassette, R |
| P5 | TATCAGAGGTAGTTGGCGTC | aadA cassette, F |
| P6 | TGTCTAGCTTCAAGTATGACG | aadA cassette, R |
| P7 | CATATGGAAACCAAAATTAATTCAC | Complementation, F |
| P8 | CTGCAGTTATTTACTTTTTTGTAATTG | Complementation, R |
| P9 | GGATCCTAATACCCGAGCTTCAAG | Complementation, PflgB, F |
| P10 | CATATGACCTCCCTCATTTAAAATTGC | Complementation, PflgB, R |
| P11 | ACCCAATATCCTAAAACTTC | RT-PCR, bb0776, F |
| P12 | TGGCAGCTGTATAAATTCCT | RT-PCR, bb0775, R |
| P13 | ACATCTGGCAAAGCACAAGA | RT-PCR, bb0775, F |
| P14 | GTATTGTTGTGCAGTCATTC | RT-PCR, bb0774, R |
| P15 | ACTCAAAAGCTATTCAAACT | RT-PCR, bb0774, F |
| P16 | TGAATTGTTGCTTTTTACCT | RT-PCR, bb0773, R |
| P17 | CACAACAAGAATAAATGATG | RT-PCR, bb0773, F |
| P18 | GTTGAAGCTTTGTTTGTTTG | RT-PCR, bb0772, R |
| P19 | TGGAGGAAATTGATGGAAAC | RT-PCR, bb0772, F |
| P20 | TTAAATCGGCAAGGCCAAAG | RT-PCR, bb0858, R |
| P21 | GCCTTGCCGATTTAATTTAC | RT-PCR, bb0858, F |
| P22 | TAGCTTGTGTTCTCTTACTG | RT-PCR, bb0771, R |
| P23 | TCATCTGCTATGATTATGCCACC | qRT-PCR, flaA, F |
| P24 | AGAATAAGCATATTCCATGCCAT | qRT-PCR, flaA, R |
| P25 | CATATTCAGATGCAGACAGAGG | qRT-PCR, flaB, F |
| P26 | CCCTGAAAGTGATGCTGGTGTG | qRT-PCR, flaB, R |
| P27 | TTCTCGCCCTACTGATGCTC | qRT-PCR, flgE, F |
| P28 | CCGTTGCCACTAACTCCAAG | qRT-PCR, flgE, R |
| P29 | AACAGGAATTAACGAGGCTG | qRT-PCR, eno, F |
| P30 | AAATTGCATTAGCACCAAGC | qRT-PCR, eno, R |
| P31 | CACCATGGAAACCAAAATTAATTCAC | rFlgJBb, F |
| P32 | TTTACTTTTTTGTAATTG | rFlgJBb, R |
a
The underlined sequences are engineered restriction cut sites for DNA cloning. F, forward; R, reverse.