Fig 4.

Copurification of CsoS1D with CsoS1 during sucrose gradient centrifugation of P. marinus MED4 carboxysomes. Aliquots of all gradient fractions (top to bottom indicated by horizontal arrow) were subjected to SDS-PAGE. CsoS1 and CsoS1D were identified by immunoblotting with their respective antibodies. Vertical arrows indicate peak chemiluminescence signals. H. neapolitanus carboxysomes (Hn) and recombinant P. marinus MED4 CsoS1D (r) were included as positive controls. M, molecular mass ladder (kDa).