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Association of CsoS1D with the P. marinus MED4 carboxysome shell. Carboxysomes (C) were incubated in turnip crinkle virus buffer (see Materials and Methods) and fractionated into a shell-associated pellet (P) and a free-protein-enriched supernatant (S) by centrifugation. CbbL, CsoS1 (lower arrow on stained gel), and CsoS1D (upper arrow on stained gel) polypeptides were identified by immunoblotting as described for Fig. 2 and 4. M, molecular mass ladder (kDa).
