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G27 ΔpurA and ΔpurB mutants are capable of growth only in the presence of specific purine bases or nucleosides. OD600 values for 24-h growth assays of H. pylori ΔpurA (A), ΔpurB (B), and ΔpurD (C) strains in defined RnP medium containing only single purine bases or nucleoside sources are shown. Starting OD600 values were between 0.03 and 0.04. Bars indicate growth of designated mutants. Error bars represent the standard errors of the means. N/A, no additive.
