Fig 2.
Man6P-containing proteins in lysosome-enriched fractions of Acp2−/−, Acp5−/−, and Acp2/Acp5−/− mice. Extracts (5 μg) of lysosome-enriched fractions from wild-type, Acp2−/−, Acp5−/−, and Acp2/Acp5−/− mice were separated by SDS-PAGE and analyzed by anti-Man6P Western blotting. The lysosome-associated Lamp1 membrane protein was used as a loading control. The positions of the molecular mass marker proteins (in kilodaltons) are indicated.