Fig 7.

Colocalization of accumulating free cholesterol with Niemann-Pick type C1 (Npc1) protein in Acp2/Acp5^−/− hepatocytes. Primary cultured hepatocytes isolated from wild-type and Acp2/Acp5^−/− mice were fixed and costained with filipin (blue) (500 μg/ml) and anti-Npc1 antibody (green) (1:100) (A) or stained for Man6P (red, 1 μg/ml) and the lysosomal protease cathepsin D (Ctsd; green) (1:2,000) (B) and analyzed by confocal microscopy. In the merged pictures, yellow indicates the colocalization. Bars, 10 μm.