Fig 3.

Conserved ETS binding sites are required for the activity of the Elf-4 −10E enhancer. (A) Nucleotide sequence alignment of the conserved region of the Elf-4 −10E enhancer. The conserved ETS binding sites are marked. (B) Stable transfection assay results in 416B cells of luciferase reporter constructs containing the wild-type −10E enhancer and the −10E enhancer with different combinations of the ETS binding sites mutated. Luciferase values were normalized relative to the SV40-luciferase control vector. The means and the standard errors of the means are shown for three experiments performed in triplicate.