Table 1.
Deduced functions of ORFs in the salinomycin biosynthesis gene cluster
| ORF | No. of amino acids | Proposed functiona | Sequence similarity (protein, origin) | % identity/similarity | Accession no. |
|---|---|---|---|---|---|
| Orf11 | 572 | 3-Hydroxybutyryl-CoA dehydrogenase | StrviDRAFT_8389, Streptomyces violaceusniger Tü 4113 | 61/70 | ZP07610704 |
| Orf12 | 342 | 3-Oxoacyl-(acyl-carrier-protein) synthase III | SSNG_07461, S. treptomyces sp. C | 77/86 | ZP07291840 |
| SlnA1 | 4,925 | PKS | LasA1, Streptomyces lasaliensis | 56/68 | CAQ64686 |
| Loading | KSQ-ATa-ACP | ||||
| Module 1 | KS-ATb-DH-KR-ACP | ||||
| Module 2 | KS-ATa-DH-ER-KR-ACP | ||||
| SlnA2 | 3,917 | PKS | NanA5, Streptomyces nanchangensis NS3226 | 51/63 | AAP42859 |
| Module 3 | KS-ATp-DH-KR-ACP | ||||
| Module 4 | KS-ATa-DH-ER-KR-ACP | ||||
| SlnA3 | 2,692 | PKS | MeiA2, S. nanchangensis NS3226 | 52/64 | ADC45534 |
| Module 5 | KS-ATa-ACP | ||||
| Module 6 | KS-ATa-KR-ACP | ||||
| SlnA4 | 1,642 | PKS | NigAVI, S. violaceusniger DSM4137 | 51/62 | DQ354110 |
| Module 7 | KS-ATp-KRa- ACP | ||||
| SlnA5 | 3,750 | PKS | LasAII, S. lasaliensis | 56/68 | CAQ64687 |
| Module 8 | KS-ATp-DH-ER-KR-ACP | ||||
| Module 9 | KS-ATb-KR-ACP | ||||
| SlnA6 | 1,435 | PKS | LasAVI, S. lasaliensis | 58/71 | CAQ64691 |
| Module 10 | KS-ATp-DH*- ACP | ||||
| SlnA7 | 1,644 | PKS | LasAIV, S. lasaliensis | 56/68 | CAQ64689 |
| Module 11 | KS-ATp-KR-ACP | ||||
| SlnA8 | 3,724 | PKS | MeiA1, S. nanchangensis NS3226 | 52/64 | ADC45586 |
| Module 12 | KS-ATp-KR-ACP | ||||
| Module 13 | KS-ATa-DH-ER-KR-ACP | ||||
| SlnA9 | 2,312 | PKS | AmphI, Streptomyces nodosus | 52/66 | AAK73501 |
| Module 14 | KS-ATb-KR-ACP | ||||
| KS | KS | ||||
| SlnBI | 128 | Epoxide hydrolase | NigBI, S. violaceusniger DSM4137 | 32/50 | ABC84467 |
| SlnDI | 265 | Thioesterase | MonAX, S. cinnamonensis | 50/65 | AA065810 |
| SlnM | 271 | O-Methyltransferase | TcmP, Streptomyces glaucescens | 39/59 | AAA67510 |
| SlnE | 93 | Ferredoxin | Tcs_55098_011, Streptomyces sp. ATCC 55098 | 53/70 | ADU56364 |
| SlnF | 407 | Cytochrome P450 | NigD, S. violaceusniger DSM4137 | 35/52 | ABC84463 |
| SlnTI | 319 | ABC transporter ATP-binding protein | TnrB2, S. longisporoflavus | 64/77 | CAA52012 |
| SlnTII | 546 | ABC transporter efflux pump | TnrB3, S. longisporoflavus | 39/53 | CAA52013 |
| SlnBII | 149 | Epoxide hydrolase | NigBI, S. violaceusniger DSM4137 | 47/64 | ABC84467 |
| SlnBIII | 153 | Epoxide hydrolase | NigBI, S. violaceusniger DSM4137 | 52/72 | ABC84467 |
| SlnC | 484 | Epoxidase | NigCI, S. violaceusniger DSM4137 | 51/67 | ABC84466 |
| SlnR | 906 | LuxR family transcriptional regulator | Las4, S. lasaliensis | 42/52 | CAQ64683 |
| SlnDII | 253 | Thioesterase | MonAX, S. cinnamonensis | 40/54 | AA065810 |
| Orf13 | 862 | Peptide synthetase | AcmC, Streptomyces anulatus | 48/61 | ADG27359 |
| Orf14 | 82 | Hypothetical protein | SSNG_00262, Streptomyces sp. C | 74/80 | ZP07284641 |
| Orf15 | 855 | SARP family transcriptional regulator | Sros_3791, Streptomyces roseum DSM 43021 | 33/45 | YP003339454 |
| Orf16 | 597 | Acyl-CoA synthetase | PRK09192, Streptomyces viridochromogenes | 43/54 | AEF16021 |
| Orf17 | 572 | Acyl-CoA dehydrogenase | SSCG_02872, Streptomyces clavuligerus ATCC 27064 | 40/52 | ZP05005545 |
| Orf18 | 96 | Peptidyl carrier protein | SSCG_02873, S. clavuligerus ATCC 27064 | 58/75 | ZP05005546 |
| Orf19 | 279 | 4′-Phosphopantetheinyl transferase | SCLAV_0457, S. clavuligerus ATCC 27064 | 50/61 | ZP06769934 |
a
*, redundant domain; a, inactive domain; ER, enoyl reductase.