Table 1.
Condition | Target DNA | Cell |
DNA |
|||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
N0 (gc ml−1)a |
k1 (h−1) |
N1 (gc ml−1) |
k2 (h−1) |
t1 (h) | R2 | T99c (h) | N0 (gc ml−1) |
k1 (h−1) |
t1 (h) | R2 | T99 (h) | |||||
Mean | SEb | Mean | SE | Mean | SE | |||||||||||
Light | Universal Bacteroidales | 1.63 × 108 | 0.245 | 0.016 | 5.15 × 105 | 0.028 | 0.005 | 0.98 | 22 | 3.79 × 108 | 0.050 | 0.008 | 0.91 | 98 | ||
Human Bacteroidales | 4.38 × 107 | 0.187 | 0.026 | 2.64 × 106 | 0.052 | 0.016 | 0.95 | 25 | 1.07 × 108 | 0.048 | 0.006 | 0.99 | 103 | |||
Cow Bacteroidales | 3.47 × 106 | 0.234 | 0.021 | 6.40 × 103 | 0.012 | 0.006 | 0.98 | 20 | 9.84 × 106 | 0.030 | 0.010 | 0.89 | 151 | |||
Dog Bacteroidales | 3.63 × 106 | 0.228 | 0.032 | 1.77 × 104 | 0.021 | 0.007 | 0.88 | 20 | 8.59 × 106 | 0.049 | 0.003 | 0.98 | 102 | |||
Cultivable Enterococcus | 3.12 × 106 | 0.081 | 0.006 | 0.94 | 56 | |||||||||||
Enterococcus | 3.21 × 106 | 0.038 | 0.006 | 0.84 | 118 | 2.65 × 107 | 0.090 | 0.002 | 23 | 0.89 | 98 | |||||
Campylobacter jejuni | 3.19 × 104 | 0.174 | 0.021 | 3.19 × 103 | 0.045 | 0.01 | 0.81 | 34 | 2.20 × 105 | 0.055 | 0.013 | 46 | 0.90 | 123 | ||
Salmonella Typhimurium | 4.42 × 105 | 0.052 | 0.01 | 23 | 0.84 | 122 | 4.13 × 106 | 0.047 | 0.002 | 23 | 0.94 | 129 | ||||
Adenovirus | 5.43 × 104 | 0.056 | 0.010 | 0.76 | 72 | |||||||||||
Dark | Universal Bacteroidales | 1.54 × 108 | 0.082 | 0.016 | 1.00 × 107 | 0.031 | 0.01 | 0.99 | 57 | 4.01 × 108 | 0.042 | 0.006 | 0.89 | 101 | ||
Human Bacteroidales | 4.62 × 107 | 0.071 | 0.011 | 2.53 × 105 | 0.025 | 0.017 | 0.95 | 62 | 1.08 × 108 | 0.059 | 0.005 | 0.98 | 95 | |||
Cow Bacteroidales | 3.40 × 106 | 0.077 | 0.008 | 3.85 × 104 | 0.012 | 0.006 | 0.95 | 59 | 1.09 × 107 | 0.045 | 0.003 | 0.87 | 125 | |||
Dog Bacteroidales | 3.60 × 106 | 0.098 | 0.011 | 1.77 × 104 | 0.021 | 0.007 | 0.95 | 47 | 9.01 × 106 | 0.050 | 0.003 | 0.97 | 91 | |||
Cultivable Enterococcus | 7.28 × 106 | 0.087 | 0.006 | 23 | 0.96 | 99 | ||||||||||
Enterococcus | 2.65 × 107 | 0.105 | 0.001 | 23 | 0.99 | 90 | 5.89 × 107 | 0.093 | 0.008 | 23 | 0.82 | 95 | ||||
Campylobacter jejuni | 3.65 × 104 | 0.041 | 0.003 | 0.97 | 121 | 2.20 × 105 | 0.064 | 0.017 | 46 | 0.88 | 118 | |||||
Salmonella Typhimurium | 3.39 × 106 | 0.041 | 0.006 | 23 | 0.96 | 144 | 6.37 × 106 | 0.043 | 0.007 | 23 | 0.97 | 154 | ||||
Adenovirus | 5.43 × 104 | 0.049 | 0.007 | 0.84 | 99 |
gc ml−1 = number of gene copies per milliliter.
SE, standard error.
Time for 2-log reduction.
The models are as follows: , where N is number of gene copies ml−1 of target DNA at time t, N0 and N1 are the initial concentration of target DNA, and k1 and k2 are the decay rate constants; , where N is number of gene copies or bacteria ml−1 at time t, N0 is the initial concentration, and k1 is the decay rate constant; and , where t1 is time at the end of lag period, t is time at any point after a lag period, and other parameters are as given in the text.