Table 1.

Sequences of the four combinations of allele-specific probes and primers used for quantification of the erg27 alleles

Gene	Codon	Allele-specific reverse primer sequence (5′ to 3′)a	Probe sequence (5′ to 3′)
erg27WT	TTC	CCATCCATCTTACAAGGTCGAAG	FAM-TTATCTACAGATTGATCTTC-MGB-NFQ
erg27F412S	TCC	CCATCCATCTTACAAGGTCGG	FAM-TTTATCTACAGATTGATCTCC-MGB-NFQ
erg27F412I	ATC	CCATCCATCTTACAAGGTCGATG	FAM-TTATCTACAGATTGATCATC-MGB-NFQ
erg27F412V	GTC	CATCCATCTTACAAGGTCGACG	FAM-TTTATCTACAGATTGATCGTC-MGB-NFQ

^aAdditional mismatches in primers are underlined, and allele-specific nucleotides are shown in boldface.