Table 2.

IL^a for the 12 possible nonspecific amplifications encountered in ASPPAA for erg27^F412 mutations (qPCR threshold at 0.15)

Gene	IL (%) for:
	erg27WT	erg27F412S	erg27F412I	erg27F412V
erg27WT		0.026	0.0036	0.0006
erg27F412S	0.0004		0.0005	0.0045
erg27F412I	0.0481	0.0001		0.1448
erg27F412V	0.0011	0.0001	0.0111

^aIL = 100/(2^{Cqs − Cqlim} + 1) according to reference 9 (n = 9 observations); see the text for further explanations.