Fig 5.

Agarose gel image of PCR amplification designed to examine the KB-1 viral metagenomic DNA sample for purity. In-house tceA primers were used (see Table S8 in the supplemental material). Lane numbers correspond to the following primer sets and samples: 1, Fermentas 1-kb ladder; 2 to 4, general bacterial 16S rRNA genes; 5 to 7, tceA gene (A); 8 to 10, hypothetical protein (hyp. pr.) gene outside putative phage genome boundary (B); 11 to 13, phage integrase gene (C); 14 to 16, hypothetical protein gene within putative phage genome boundary (D); 17 to 19, bacteriophage capsid protein gene (E); 20 to 22, bacteriophage tail protein gene (F). For all primer sets, a no-template control (−), a KB-1 community gDNA sample (+), and the KB-1 viral metagenomic DNA (V) were assayed. Letters A to F correspond to the letters identifying these genes in Fig. 4.