Table 1.
Population structure of KB-1 and estimated coverage of the different genomes on the shotgun arraya
| KB-1 OTUb | % of population |
Representative genome size (Mb) | Estimated depth of genome coverage on array |
||
|---|---|---|---|---|---|
| Low | High | Low | High | ||
| Dehalococcoides | 48.1 | 70.2 | 1.4 | 12.5 | 18.2 |
| Chlorobi SJA-28 | 6.0 | 14.9 | 2.6 | 0.8 | 2.1 |
| Acetobacterium | 2.7 | 12.0 | 3.3 | 0.3 | 1.3 |
| Spirochaetes SA-8 | 2.8 | 8.3 | 2.8 | 0.4 | 1.1 |
| Sporomusa | 1.2 | 11.5 | 4.4 | 0.1 | 0.9 |
| Methanomicrobiales | 3.5 | 5.8 | 2.7 | 0.5 | 0.8 |
| OP5 | 2.5 | 5.0 | 3.2 | 0.3 | 0.6 |
| Geobacter | 3.0 | 4.4 | 4.4 | 0.2 | 0.4 |
| Methanosarcina | 0.1 | 1.0 | 4.5 | <0.01 | 0.1 |
| Bacteroidales 1 | <0.1 | 0.8 | 5.5 | <0.01 | 0.1 |
| Syntrophobacter | <0.1 | 0.2 | 5.0 | <0.01 | <0.1 |
a
Microorganisms that were detected in KB-1 by 16S rRNA genes are listed. Low and high estimates of the percentages of the population represent the range of abundance measured by qPCR over the time period that the arrays were constructed and experiments were conducted (see reference 37 for methods). The genome coverage is based on an average amplicon size of 2 kb for 18,042 amplicons. The representative genome size is an average from closely related organisms.
b
OTU, operational taxonomic unit.