Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description and relevant feature(s)a | Source or reference |
|---|---|---|
| Strains | ||
| D. vulgaris Hildenborough | Wild type; NCIMB 8303 | 19 |
| D. vulgaris Hildenborough JW9007 | ΔDVU0379, Kmr | This study |
| D. vulgaris Hildenborough JW9009 | ΔDVU2097, Kmr | This study |
| D. vulgaris Hildenborough JW9011 | ΔDVU2547, Kmr | This study |
| D. vulgaris Hildenborough JW9013 | ΔDVU3111, Kmr | This study |
| Plasmids | ||
| pMO719 | Desulfovibrio shuttle vector; SRB replicon pBG1 (EcoRI-EcoRI from pSC27) ligated to the EcoRI-bounded pCR8/GW/TOPO fragment; Specr | 22 |
| pMO719-DVU0379 | Complementation plasmid for JW9007; DNA fragment 425654-429479 (3,826 bp) cloned into the EcoRV site in pMO719; chromosome position of DVU0379, 428780-429457; Specr | This study |
| pMO719-DVU2097 | Complementation plasmid for JW9009; DNA fragment 2185067-2186208 (1,142 bp) cloned into the EcoRV site in pMO719; chromosome position of DVU2097, 2185336-2186007; Specr | This study |
| pMO719-DVU2547 | Complementation plasmid for JW9011; DNA fragment 2659090-2660860 (1,771 bp) cloned into the EcoRV site in pMO719; chromosome position of DVU2547, 2659978-2660664; Specr | This study |
| pMO719-DVU3111 | Complementation plasmid for JW9013; DNA fragment 3260525-3261583 (1,059 bp) cloned into the EcoRV site in pMO719; chromosome position of DVU3111, 3260648-3261382; Specr | This study |
a
Kmr, kanamycin resistance; Specr, spectinomycin resistance.