Fig 1.

(A) Structures of linear substrates of Z. mobilis ZMO1548-Shc and their cyclization products. All isomers except for neoiso-isopulegol were detected as cyclization products from (R,S)-citronellal with ZMO1548-Shc as a catalyst. neoiso-Isopulegol is given for the completeness of isomer/enantiomer structures. (B) Cyclization of citronellal to isopulegol (top) and squalene to hopene (bottom) by Z. mobilis SHCs. Extracted starting material and products were analyzed by GC and/or by GC-MS. Error bars indicate standard deviations. Experiments were performed with at least three independent batches of E. coli cells (3 biological replicates and 12 technical replicates). The 100% value corresponds to 20 mM starting material.