Table 1.
Sample | Gd-DTPA | ProCA1 | ProCA1 PEG0.6k |
ProCA1 PEG2.4k |
ProCA1 PEG12k |
---|---|---|---|---|---|
D | - | 13.9 | 12.9 | 12.1 | 6.5 |
Radius (Å) | - | 16.9 | 18.2 | 19.4 | 40.2 |
Log Ka | 18.4[11] | 11.46 | 11.26 | 11.70 | 11.08 |
q | 1.1[30] | 2.4 | 3.2 | 3.0 | 2.6 |
r1 (mM−1 s−1) | 4.5 | 23.8 | 39.5 | 47.6 | 83.8 |
r2 (mM−1 s−1) | 5.2 | 43.7 | 92.5 | 98.7 | 100.8 |
D is the diffusion constant (×107 cm2 s−1) calculated from the Debye-Stokes-Einstein equation. The radius values were obtained by fitted data from the pulse-field-gradient NMR experiment. q is the water coordination number determined by measuring Tb3+ luminescence decay in H2O or D2O. Ka is the conditional association constant of contrast agents with Gd3+ and is the reciprocal of dissociation constant Kd (Kd = 1/Ka). Relaxation times were measured on the 0.47 T Mini Spec Relaxometer (Bruker).