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. Author manuscript; available in PMC: 2012 Dec 23.
Published in final edited form as: Chem Biol. 2011 Dec 23;18(12):1649–1657. doi: 10.1016/j.chembiol.2011.09.019

Figure 5. Burst kinetic profiles with WT and R218K luciferases.

Figure 5

Purified luciferases (10 nM) were rapidly injected at the 10-second time point into 10 μM of CycLuc1 (A), CycLuc2 (B), or 6’-Me2NLH2 (C) and the light emission was recorded. Burst emission behavior for all substrates and characterized mutants is detailed in Figure S3, with emission wavelengths reported in Table S3.