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. 2011 Dec 15;106(3):517–524. doi: 10.1038/bjc.2011.553

Figure 3.

Figure 3

Moleculocytogenetic specification of chromosome 12 and NAV3 aberrations in colon cancer cell lines. The aberrant cells of lines CCL-230 (A–G) and CRL-2577 (H–J) most commonly showed three copies of chromosome 12 (A, H: centromere 12 green), with loss of NAV3 by specific bacterial artificial chromosome (BAC)-probes in one of them (A: RP11-36P3 red, H: RP11-136F16 red). In the cell line CCL-230, a translocation from chromosome 15 (B, C: red; G: wine-red in arm-MFISH) to chromosome 12 (B and G: green) was specified to 12p (G: orange), and 12q showed a deletion (D–F: shortened q-arm and change of the inverted DAPI banding pattern). In the cell line CRL-2577, an unbalanced translocation between chromosomes 12 (I: red, MFISH) and 2 (I, J: blue, MFISH) abolished the NAV3 gene. The line CLL-248 mostly showed five copies of chromosome 12, one of them missing NAV3 (K: centromere 12 blue, BAC RP11-36P3, orange), but no translocations in arm-MFISH (L). The deletion extended to the regions defined by YAC probes 825F9 (M) and 885G4 (N). In the line CLL-228, (O–T) the NAV3- specific signal (RP11-36P3, red) was seen in one abnormal chromosome (O) and in the normal chromosome 12 (Sub-figure Q), but not in two other chromosomes with centromere 12 (Sub-figures P, Q: centromere 12 green), nor in the minute chromosomes with centromere 12 material (Sub-figure Q: green). The corresponding aberrant chromosomes by arm-MFISH (R–T) are t(2;12) (R: blue; orange), der(10)t(10;12; 3) (S: blue; orange; green) and i(12)(p) (T: orange, the aberrant chromosome is on the right, a normal chromosome 12 is on the left). YAC-and BAC probes as published previously (12).