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. 2011 Dec 2;31(3):593–605. doi: 10.1038/emboj.2011.431

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Copyright © 2012, European Molecular Biology Organization

This is an open-access article distributed under the terms of the Creative Commons Attribution Noncommercial No Derivative Works 3.0 Unported License, which permits distribution and reproduction in any medium, provided the original author and source are credited. This license does not permit commercial exploitation or the creation of derivative works without specific permission.

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Effect of acetylation on recruitment of PRC2 to chromatin. (A) ChIP for H3K27ac and IgG control in wild-type cells treated with TSA and in Mbd3^−/− cells without TSA treatment. (B) Relative enrichment for Mi2β and IgG control in wild-type cells treated with TSA compared with untreated. (C) Relative enrichment for Suz12 and IgG control in wild-type cells treated with TSA compared with untreated. Error bars show s.e.m. (D) Western blot of nuclear extracts prepared from wild-type ES cells with or without TSA treatment. Alpha tubulin is used as a loading control.