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. 2011 Nov 29;31(3):754–766. doi: 10.1038/emboj.2011.429

Figure 6.

Figure 6

PI4KB interacts with ACBD3. (A) The mammalian two-hybrid assay was performed to examine interactions between PI4KB and ACBD3 or the virus proteins and the results are represented as in Figure 1A. (B) Co-immunoprecipitation of PI4KB with ACBD3. HA-tagged PI4KB was co-expressed with FLAG-tagged ACBD3 or FLAG-tagged 3A in 293T cells, and the subsequent analysis was performed as in Figure 1B. (C) Mapping of the PI4KB-binding region of ACBD3. Schematic representation of full-length ACBD3 (WT) and its mutants (mut6 and mut7) (upper panel). Mammalian two-hybrid analysis was performed to examine interactions between PI4KB and ACBD3 WT or its mutants (mut1–7), and the results are represented as described in Figure 1A (lower panel). Figure source data can be found in Supplementary data.