Table 2.
Effects of plant SPO11 expression on fertility in the Drosophila mei-W681 (dmspo11-deficient) homozygote
|
mei-W681 hetero- zygosity |
Transgene | Mothers crossed | Total eggs laid | Pupae generated | % Fertility |
|---|---|---|---|---|---|
| +/1 | none | 37 | 3768 | 3309 | 88 |
| 1/1 | none | 107 | 6109 | 1568 | 26 |
| 1/1 | mei-W68+ § | 11 | 1207 | 1047 | 87 |
| 1/1 | AtSPO11-1a | 40 | 2880 | 691 | 24 |
| 1/1 | AtSPO11-2b | 20 | 1536 | 359 | 23 |
| 1/1 | OsSPO11Dc | 60 | 3577 | 753 | 21 |
Every female (w/w) was crossed to a few males of Canton-S strain (w+ /Y). The numbers of total eggs laid and the pupae subsequently generated were scored. Fertility was calculated by dividing the number of pupae (representing viable eggs) by the number of total eggs. Transgenes are indicated as Vector name{gene, insertion #}.
§: P{hsp83-mei-W68+ cDNA, M53-3}
a: P{hsp83-AtSPO11-1 cDNA, A5-1}
b: P{hsp83-AtSPO11-2 cDNA, 2M3-1}
c: P{hsp83-OsSPO11D cDNA, F2-1}