Table 3.
Effects of plant SPO11 expression on X chromosome nondisjunction in the Drosophila mei-W681 homozygote
| Transgene of mei-W681 homozygote | Mothers crossed | Total progeny scored | Nullo-X eggs (%) |
Diplo-X eggs (%) |
Nullo-X eggs without associated diplo-X eggs (%) |
P value from chi test |
|---|---|---|---|---|---|---|
| Exp. 1 | ||||||
| None | 99 | 1283 | 146 (11.4) | 106 (8.3) | 40 (3.1) | - |
| AtSPO11-1a | 74 | 1204 | 138 (11.5) | 95 (7.9) | 43 (3.6) | 5.3E-01 |
| AtSPO11-2b | 71 | 1187 | 178 (15.0) | 88(7.4) | 90 ** (7.6) | 6.9E-07 |
| OsSPO11Dc | 97 | 1384 | 184 (13.3) | 106 (7.7) | 78 ** (5.6) | 1.6E-03 |
| Exp. 2 | ||||||
| none | 55 | 1245 | 119 (9.6) | 88 (7.1) | 31 (2.5) | - |
| OsSPO11DY213Fd | 57 | 1994 | 175 (8.8) | 129 (6.5) | 46 (2.3) | 7.4E-01 |
| mei-W68+ e | 22 | 3434 | 3 (0.09) | 3 (0.09) | 0 (0) | - |
Every female (w/w) was crossed to a few males of Canton-S strain (w+ /Y). Nullo-X and diplo-X eggs were detected as w+ sons (X0) and as w daughters (XXY), respectively. The number of nullo-X eggs without associated diplo-X eggs was calculated by subtracting the number of diplo-X eggs from the number of nullo-X eggs. The number of diplo-X eggs was used as the expected value for the number of nullo-X eggs with associated diplo-X eggs, which were generated by chromosome nondisjunction during the first meiotic division. The egg laying periods were 5 days and 10 days in Exp. 1 and Exp. 2, respectively. Transgenes are indicated as Vector name{gene, insertion #}.
a: P{hsp83-AtSPO11-1 cDNA, A5-1}
b: P{hsp83-AtSPO11-2 cDNA, 2M3-1}
c: P{hsp83-OsSPO11D cDNA, F2-1}
d: P{hsp83-OsSPO11DY213F, F22-F2}
e: P{hsp83-mei-W68+ cDNA, M53-3}
***: P < 1E-03; **: 1E-03 <P < 1E-02; *: 1E-02 <P < 5E-02.