. 2012 Feb 6;7(2):e31390. doi: 10.1371/journal.pone.0031390

Table 2. Basal plasma cholesterol concentrations of sows at 5 days before and 24, 66 and 108 days after insemination.

		Diet¹						P value²
	T	LP	SE	AP	SE	HP	SE	D	R	T	D×R	D×T
No. of animals		16		17		15
Total cholesterol, mmol/l
	−5	2.23^A	0.11	2.64^A	0.11	2.48^A	0.12	0.091	<0.001	<0.001	0.360	0.066
	24	1.77^B	0.10	2.14^B	0.11	1.93^B	0.11
	66	1.94^A	0.11	1.92^B	0.11	2.20^A	0.11
	108	1.81^B	0.11	1.81^B	0.11	1.81^B	0.11
LDL cholesterol, mmol/l
	−5	0.79	0.09	1.03	0.10	0.82	0.10	0.002	0.261	0.343	0.917	0.060
	24	0.50^b	0.09	0.96^a	0.09	0.88^ab	0.09
	66	0.68	0.09	0.71	0.09	0.97	0.09
	108	0.59	0.09	0.70	0.09	0.85	0.09
HDL cholesterol, mmol/l
	−5	1.32^A	0.06	1.51^A	0.06	1.42^A	0.06	0.015	<0.001	<0.001	0.354	0.071
	24	0.95^ab ^, ^B	0.06	1.06^a ^, ^B	0.06	0.77^b ^, ^B	0.06
	66	0.99^B	0.06	0.96^B	0.06	0.83^B	0.06
	108	0.96^B	0.06	0.87^B	0.06	0.78^B	0.06
HDL cholesterol, %
	−5	60.5^A	2.8	57.8^A	2.8	60.0^A	3.0	<0.001	0.001	<0.001	0.085	0.035
	24	54.4^a ^, ^B	2.5	48.2^a,b ^, ^B	2.5	39.9^b ^, ^B	2.7
	66	50.2^a ^, ^B	2.5	52.2^a ^, ^B	2.5	37.2^b ^, ^B	2.7
	108	54.4^B	2.6	50.9^a ^, ^B	2.5	42.5^B	2.7

Sows were fed isoenergetic diets with low protein∶high carbohydrate (6.5% crude protein, LP), adequate protein∶carbohydrate (12.1% crude protein, AP), and high protein∶low carbohydrate (30% crude protein, HP) ratios throughout pregnancy (EXP1).

^a–c

Within a row, values not sharing a common superscript differ significantly (P<0.05) according to Tukey post hoc test.

^A–C

Within diet and variable, values not sharing a common superscript differ significantly (P<0.05) according to Tukey post hoc test.

Values are least squares means ± SE per diet over 7 replicates.

ANOVA PROC MIXED, D = diet, R = replicate, T = time in days after insemination. D×R = interaction of diet×replicate. D×T = interaction of diet×time.