. 2012 Feb 6;7(2):e31390. doi: 10.1371/journal.pone.0031390

Table 3. Basal plasma metabolite concentrations of sows at 5 days before and 24, 66 and 108 days after insemination.

		Diet¹						P value²
	T	LP	SE	AP	SE	HP	SE	D	R	T	D×R	D×T
No. of animals		16		17		15
Glucose, mmol/l
	−5	4.24	0.13	4.14	0.12	4.02	0.14	0.407	0.036	0.001	0.316	0.898
	24	3.95	0.12	3.99	0.12	3.94	0.13
	66	3.87	0.12	3.65	0.12	3.76	0.13
	108	4.24	0.12	4.03	0.12	4.20	0.13
NEFA, mmol/l
	−5	0.41	0.21	0.43	0.20	0.56	0.22	0.623	0.018	<0.001	0.129	0.917
	24	0.54	0.19	0.92	0.18	0.76	0.20
	66	0.89	0.19	1.00	0.18	0.99	0.20
	108	1.63	0.20	1.73	0.18	1.74	0.20
Triacylglycerol, mmol/l
	−5	0.29^A	0.04	0.26^A	0.04	0.22^A	0.04	0.910	0.402	<0.001	0.775	0.013
	24	0.35^A	0.05	0.33^A	0.04	0.35^B	0.04
	66	0.40^B	0.04	0.35^B	0.04	0.34^B	0.04
	108	0.57^B	0.04	0.64^B	0.04	0.72^C	0.04
Urea, mmol/l
	−5	4.6^A	0.2	4.3^A	0.2	4.9^A	0.3	<0.001	0.011	<0.001	0.006	<0.001
	24	1.4^c ^, ^B	0.2	2.6^b ^, ^B	0.2	6.9^a ^, ^B	0.2
	66	1.5^c ^, ^B	0.2	2.5^b ^, ^B	0.2	6.3^a ^, ^B	0.2
	108	1.7^c ^, ^B	0.2	3.0^b ^, ^B	0.2	7.1^a ^, ^B	0.2
Serum protein, mg/ml
	−5	77.4^A	1.3	78.4	1.2	78.6	1.4	0.002	<0.001	<0.001	0.607	0.002
	24	73.5^A	1.2	76.7	1.1	75.2	1.3
	66	75.6^A	1.2	80.2^A	1.1	78.3	1.3
	108	67.6^b ^, ^B	1.2	75.1^a ^, ^B	1.1	77.2^a	1.3

Sows were fed isoenergetic diets with low protein∶high carbohydrate (6.5% crude protein, LP), adequate protein∶carbohydrate (12.1% crude protein, AP), and high protein∶low carbohydrate (30% crude protein, HP) ratios throughout pregnancy (EXP1).

^a–c

Within a row, values not sharing a common superscript differ significantly (P<0.05) according to Tukey post hoc test.

^A–C

Within diet and variable, values not sharing a common superscript differ significantly (P<0.05) according to Tukey post hoc test.

Values are least squares means ± SE per diet over 7 replicates.

ANOVA PROC MIXED, D = diet, R = replicate, T = time in days after insemination. D×R = interaction of diet×replicate. D×T = interaction of diet×time.