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Immunofluorescence of the carotid plaque to detect the presence of (A) HN with (B) CD68. (C) Nuclei can be visualized using a DAPI counterstain. (D) Merging of these images demonstrates co-localization of HN with plaque macrophages, (E) seen better with increased magnification. Merged images below demonstrate co-localization of HN with (F) smooth muscle α-actinin, (G) fibroblast vimentin, and (H) dendritic cell fascin.
