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. 2012 Feb 6;7(2):e31066. doi: 10.1371/journal.pone.0031066

Figure 1. Complement-binding capacity of BP180/CXVII-specific rabbit antibodies.

Figure 1

Frozen murine skin sections were incubated with rabbit antibodies and subsequently with fresh human serum as a source of complement. Bound C3 was visualized at the dermal-epidermal junction by fluorochrome-labeled antibody. (A) Deposits of C3 in sections incubated with BP180/CXVII-specific rabbit antibody. (B) No C3 deposition in sections incubated with normal rabbit IgG (magnification, ×400).