Figure 4. RFC140/flp exon-intron organization (A) and intergenic region sequence (B).
The RFC140 gene exons are shown in black. The single flp exon and 3′ UTR are shown in red and white respectively. The coding sequence of the RFC140 gene has been deposited in GenBank under accession number FJ154842. Annotations in Panel B are as follows: gray, RFC140 stop codon and flp start codon; green, putative polyadenylation signal; double-underline, T-rich sequence; single-underline, polypyrimidine tract associated with the trans-splicing acceptor dinucleotide; blue, trans-splicing acceptor dinucleotide; yellow, additional AG dinucleotides changed to AA in mutant plasmid.