Table 2.
Immunomediators present in the supernatants of THP-1 cells expressing DENV NS proteins.
| IFNγ
|
VEGF
|
IP-10
|
TNFα
|
IL-6
|
IL-8
|
|||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Meana |
SDb |
Mean
|
SD
|
Mean
|
SD
|
Mean
|
SD
|
Mean
|
SD
|
Mean
|
SD
|
|
| No pDNA supernatantc | 1.7 | 0.5 | 17 | 4.7 | 13 | 3.9 | 4.4 | 1.8 | 1.9 | 0.7 | 241 | 116 |
| V5 supernatantd | 1.5 | 0.4 | 18 | 6.4 | 14 | 5.6 | 5.3 | 3.8 | 2.5 | 1.4 | 202 | 65 |
| NS3B3(pro) supernatantd | 1.8 | 0.2 | 22 | 1.4 | 10 | 6.2 | 11 | 7.8 | 2.8 | 0.9 | 218 | 145 |
| NS3 supernatantd | 1.4 | 0.4 | 18 | 7.1 | 12 | 7.1 | 14 | 7.3 | 2.2 | 0.6 | 319 | 130 |
| NS4AB supernatantd | 1.3 | 0.6 | 20 | 3.5 | 9.6 | 6.4 | 12 | 5.6 | 2.3 | 0.4 | 229 | 91 |
| NS4B supernatantd | 3.4 | 1.2 | 19 | 13 | 44* | 9.7 | 2.7 | 9.5 | 57* | 17 | 1,100* | 449 |
| 2KNS4B supernatantd | 6.5* | 0.7 | 46 | 11 | 65* | 15 | 30 | 13 | 185§ | 36 | 2,413§ | 382 |
| NS5 supernatantd | 5.6* | 1.0 | 40 | 14 | 59* | 13 | 29 | 11 | 109* | 30 | 1,513* | 393 |
| LPS supernatant | 6.2 | 0.9 | 20 | 8.9 | 63 | 9.9 | 115 | 12 | 223 | 19 | 3,109 | 267 |
a
pg/ml n ≥ 6 samples from at least 3 experiments
b
SD = standard deviation
c
Supernatant from THP-1 cells electroporated without pDNA
d
Supernatant from THP-1 cells electroporated with specified pDNA
*
Statistical significance (p < 0.05) compared to V5 pDNA control
§
Statistical significance (p < 0.05) compared to NS5 pDNA sample