Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2013 Jan 20.

Published in final edited form as: Virology. 2011 Nov 29;422(2):326–337. doi: 10.1016/j.virol.2011.10.030

Table 4.

Primers employed for the analysis of DENV and host genes by qRT-PCR.

Gene (GenBank no.)	Primer Sequence (5’ - 3’)	Tm (°C)	Amplicon Size (bp)
DENV Capsid^a

Forward	CAATATGCTGAAACGCGAGAG	60	167
Reverse	CATCTATTCAGAATCCCTGCT	60	167

ICAM-1 (NM_002162)^b

Forward	AGCGGCAGTTACCATGTTAGGG	58	146
Reverse	GGCTTTATTGGTGCGGAATCTGA	58	146

VCAM-1(BC068490)^b

Forward	TGCTGCTCAGATTGGAGACTC	55	113
Reverse	TCCTCACCTTCCCGCTCAG	55	113

E-selectin (NM_000450)^b

Forward	TGAGACAGAGGCAGCAGTG	57	199
Reverse	CCGTGGAGGTGTTGTAAGAC	57	199

GAPDH (BC025925)^b

Forward	AGTTAGCCGCATCTTCTTTTGC	57	96
Reverse	CAATACGACCAAATCCGTTGACT	57	96

^a

Real-time PCR primers to determine DENV2 NGC copy numbers

^b

Real-time RT-PCR primers to amplify adhesion molecule genes or GAPDH endogenous control