Fig. 6.

Analysis of interaction of the wild type HspB1 and HspB6 (a) and HspB5 and HspB6 (b) by means of analytical ultracentrifugation. Distribution of sedimentation coefficients of isolated HspB6 (dash-dotted curve 1), of isolated HspB1 and HspB5 (dash-dotted curves 2 on (a, b), respectively), of the unheated mixture of two small heat shock proteins (dashed curve 3) and of the heated mixture of two corresponding small heat shock proteins (solid curve 4) is presented on each panel. Insert on (b) represents enlarge part of sedimentogram in the range of 6–22 S