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. Author manuscript; available in PMC: 2013 Feb 15.
Published in final edited form as: J Immunol. 2012 Jan 16;188(4):1630–1637. doi: 10.4049/jimmunol.1101712

Fig. 1.

Fig. 1

Nrf2 inhibits IFNγ mRNA expression and protein secretion in splenocytes activated with anti-CD3/anti-CD28. Freshly-isolated splenocytes were pretreated with vehicle (VH) or tBHQ for 30 min prior to activation with anti-CD3/anti-CD28. A.) The cell supernatants were then collected after 24 h and cytokines analyzed. B.) Total RNA was isolated 6 h after activation and IFNγ mRNA expression quantified by real-time PCR. C.) Fold difference in IFNγ mRNA levels between wild-type and Nrf2-null splenocytes, 6 h after activation. * denotes p<0.05 as compared to wild-type control. † denotes p<0.05 as compared to Nrf2-null control. ‡ represents a significant difference (p<0.05) between the wild-type and Nrf2-null genotypes.