Fig. 3.
Effect of Nrf2 activation on cytokine production in activated CD4+ cells. Magnetically-isolated CD4+ cells from Nrf2-null and wild-type mice were pretreated with vehicle (VH) or tBHQ for 30 min prior to activation with anti-CD3/anti-CD28. The cells were then cultured for 96 h after which the cell supernatants were harvested. Cytokine concentrations in the supernatants were quantified by multiplex suspension assay. * denotes p<0.05 as compared to wild-type control. ‡ represents a significant difference (p<0.05) between the wild-type and Nrf2-null genotypes.