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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Biochim Biophys Acta. 2011 Oct 25;1823(2):264–272. doi: 10.1016/j.bbamcr.2011.10.005

Fig. 4.

Fig. 4

DR cells show no release of cytochrome c from the inner mitochondrial membrane space following Cr(VI) treatment. CC1, DR1and DR2 cells were treated with 6 μM Cr(VI) for 24 h. After treatment, the cells were incubated with Mitotracker CMXRos, fixed and incubated with mouse anti-cytochrome c antibody and Alexa 488-conjugated goat anti-mouse secondary antibody, and DNA was stained with Hoechst. Cells were seeded on 8-well chamber slides prior to treatment. Mitochondria – red; cytochrome c – green; nucleus– blue. All images were taken on a Zeiss 510 laser scanning microscope at a magnification level of 63 X. Scale bar represents 10 microns. The release of cytochrome c as assessed by segmentation threshold analysis of mitochondrial versus cytosolic localization was quantified using Image Pro-Plus software. Data are mean +/− SE of three experiments and are normalized to Mitotracker CMXRos.