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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: J Dermatol Sci. 2011 Oct 19;65(2):86–94. doi: 10.1016/j.jdermsci.2011.10.003

Figure 1. Hypoxia and hypoxia mimetics inhibit TNFα-induced VCAM-1.

Figure 1

(a) VCAM-1 expression (by ELISA) on HDMEC, pretreated as indicated with DMOG either 6 or 16 hr prior to TNFα (b) HDMEC pretreated with DMOG or DP for 6 hr followed by TNFα were assessed for VCAM-1 expression by western analysis (c) HDMEC were pretreated with DMOG (500 μM) for times ranging from 6 hr to 2 hr after (−2 hr) TNFα stimulation and assayed for VCAM-1 (d) Pretreatment with FG-4497 prior to TNFα stimulation (e) HDMEC were stimulated with TNFα, hypoxia alone (24 hr), hypoxia (8 hr) followed by TNFα under normoxic conditions, hypoxia (8 hr) followed by TNFα under continuous hypoxia, and TNFα under hypoxia without hypoxia pretreatment. Values above bands represent the relative density as compared to lane 2 adjusted for protein loading. (All TNFα 1000 U/mL × 16 hours – representative experiment of three experiments)