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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: J Dermatol Sci. 2011 Oct 19;65(2):86–94. doi: 10.1016/j.jdermsci.2011.10.003

Figure 3. DMOG does not inhibit TNFα-mediated nuclear translocation of NFκB, but inhibits TNFα-mediated chromatin modification.

Figure 3

(a) HDMEC were treated with TNFα (1000 U/mL × 30 min), then fixed and stained with antibodies against the p65 domain of NFκB. Bright nuclear NFκB staining persisted in DMOG (500 μM) and DP (125 μM) pretreated cells. (b) HDMEC treated with TNFα (500 U/mL × 6 hr) were evaluated by ChIP as described in materials and methods. TNFα treatment induced trimethylation and acetylation of histone H3 associated with VCAM-1 promoter elements. Pretreated with (c) DP (125 μM) or (d) DMOG (500 μM) as indicated inhibited TNFα-stimulated histone H3 modification. Diluent controls were PBS or DMSO for DMOG- and ethanol for DP-treated cells. (p-values calculated using paired Student’s two-tailed t-test).