Skip to main content
. Author manuscript; available in PMC: 2013 Feb 15.
Published in final edited form as: J Immunol. 2012 Jan 6;188(4):1970–1980. doi: 10.4049/jimmunol.1102383

Figure 1.

Figure 1

MMP-1 and MMP-3 induce macrophage expression of mPGES-1. [A] RAW264.7 macrophages (6-well plate; 2 × 106 cells/well) or [B] thioglycollate-elicited peritoneal macrophages (12-well plate; 7.5 × 105 cells/well) were incubated in DMEM-0.1% LE-BSA (Ctrl) or media containing 50nM MMP-1, MMP-3 or 10 ng/ml LPS. Following 18 h incubation, total RNA or cell lysates were recovered. Levels of mPGES-1 and mPGES-2 mRNAs were determined by PCR. Levels of cPGES and GAPDH in cell lysates were determined by Western blot. Data are representative blots and the mean levels (± SD) of target mRNAs or proteins, presented as RDUs, of 3 experiments.